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M9490071.TXT
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1994-09-03
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Document 0071
DOCN M9490071
TI Removal of the cleavage site of recombinant feline immunodeficiency
virus envelope protein facilitates incorporation of the surface
glycoprotein in immune-stimulating complexes.
DT 9411
AU Rimmelzwaan GF; Siebelink KH; Huisman RC; Moss B; Francis MJ; Osterhaus
AD; Department of Virology, Erasmus University Rotterdam, The;
Netherlands.
SO J Gen Virol. 1994 Aug;75 ( Pt 8):2097-102. Unique Identifier : AIDSLINE
MED/94322005
AB Recombinant vaccinia viruses were constructed that expressed the
complete env gene of feline immunodeficiency virus with or without the
nucleotide sequence encoding the cleavage site between the surface (SU)
protein and the transmembrane (TM) protein. The removal of this cleavage
site resulted in the expression of a 150K protein that was processed
into a 130K protein and was not cleaved into the SU and the TM proteins.
Removal of the cleavage site also facilitated incorporation of the SU
protein in immune-stimulating complexes (iscoms). Antibody responses to
both an SU and a TM peptide representing two immunodominant B cell
epitopes were measured. These were higher in cats immunized with iscoms
prepared from the cleavage site-deleted envelope protein than in cats
immunized with iscoms prepared from the native envelope protein or
immunized with the envelope protein and the adjuvant Quil A.
DE Animal Antibodies, Viral/*BIOSYNTHESIS Antigens,
Viral/GENETICS/*IMMUNOLOGY B-Lymphocytes/IMMUNOLOGY Base Sequence
Cats Feline Acquired Immunodeficiency Syndrome/PREVENTION & CONTROL
Gene Products, env/GENETICS/*IMMUNOLOGY Immunodeficiency Virus,
Feline/GENETICS/*IMMUNOLOGY Immunodominant Epitopes/IMMUNOLOGY
Molecular Sequence Data Protein Processing, Post-Translational/GENETICS
Recombinant Proteins/IMMUNOLOGY Vaccines, Synthetic/IMMUNOLOGY JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).